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For ROR2 and RhoA inhibition experiments, we treated HBVPCs at a density of 90% with Sema4D (1.6ug/mL) for 8 h in the presence or absence of anti-ROR2 (MedChemExpress, #HY-145626) (1 g/mL) or the RhoA inhibitor Rhosin hydrochloride (MedChemExpress, #HY-12646) (40 M)

While all types of e-cigarettes exerted negative effects on most of the study measures, these effects seemed to be increased in a dose-response manner with nicotine